goat anti platelet endothelial cell adhesion molecule 1 pecam 1 polyclonal antibody  (Santa Cruz Biotechnology)

Journal: Acta Histochemica et Cytochemica

Article Title: Localization of Both CD31- and Endomucin-Expressing Vessels in Mouse Dental Pulp

doi: 10.1267/ahc.24-00009

Figure Lengend Snippet: Localization of CD31 (+) and endomucin (+) vessels. ( A ) Stage of tooth crown formation at 1 week. Endomucin (+) vessels (red) mainly exist in the dental pulp. ( B ) Higher magnifications of the boxed regions in A . Both CD31(+) and endomucin (+) vessels (yellow) are narrow and located beneath the coronal dentin. ( C ) Higher magnifications of the boxed regions in B . Merged immunoreaction (yellow) is localized at thin vessels in the odontoblast layer. ( D ) Dental pulp at 4 weeks. Both CD31 (+) and endomucin (+) vessels decreased in the crown and increased in the root of dental pulp at 4 weeks. ( E ) Higher magnifications of the boxed regions in D . Thin vessels are colored in yellow, and express both CD31 (+) and endomucin (+) and located near the radicular dentin (arrowheads). ( F ) Higher magnifications of the boxed regions in E . Note the merged immunoreaction (yellow) shows mesh-like pattern. ( G ) Dental pulp at 12 weeks. Root canals at 12 week are narrower than those at 4 week. ( H ) Higher magnifications of the boxed regions in G . CD31 (+)-immunoreaction is mainly seen, and Both CD31 (+) and endomucin (+) vessels are faintly seen in dental pulp (arrowhead). ( I ) Higher magnifications of the boxed regions in H . CD31 (+) vessels are seen beneath the odontoblast layer. ( J ) Dental pulp at 56 weeks. Few CD31 (+) and endomucin (+) vessels were detected in dental pulp. ( K ) Higher magnifications of the boxed regions in J . Main vessels are positive for CD31 in the coronal pulp. ( L ) Higher magnifications of the boxed regions in K . Immuno-reaction for endomucin was separately seen at the CD31(+) vessels. Green: CD31 (+), Red: Endomucin (+), Yellow: CD31 (+) and endomucin (+). Arrowheads: CD31 (+) and endomucin (+) vessels. Den: dentin; Ob: odontoblast layer. Bars = 200 μm ( A , D , G , J ), 50 μm ( B , E , H , K ) and 15 μm ( C , F , I , L ).

Article Snippet: Sections of decalcified samples were incubated with an Alexa Fluor 488-conjugated anti-CD31/PECAM-1 goat polyclonal antibody (dilution 1:100, FAB3628G-10, R&D Systems, Minneapolis, MN, USA) and a rat monoclonal antibody against mouse endomucin (dilution 1:200, sc-65495, Santa Cruz Biotechnology Inc., Dallas, TX, USA) at 20 ± 5°C for 8 hr or 4°C overnight in the dark.

Techniques:

Journal: Acta Histochemica et Cytochemica

Article Title: Localization of Both CD31- and Endomucin-Expressing Vessels in Mouse Dental Pulp

doi: 10.1267/ahc.24-00009

Figure Lengend Snippet: Ratio of CD31 (+) and endomucin (+) vessels. Data represent means ± standard deviations (SD). Four mice were used for statistical analysis in each group. These ratios 12 and 56 weeks were significantly lower than those 1 and 4 weeks. * Statistical significance ( P < 0.05)

Article Snippet: Sections of decalcified samples were incubated with an Alexa Fluor 488-conjugated anti-CD31/PECAM-1 goat polyclonal antibody (dilution 1:100, FAB3628G-10, R&D Systems, Minneapolis, MN, USA) and a rat monoclonal antibody against mouse endomucin (dilution 1:200, sc-65495, Santa Cruz Biotechnology Inc., Dallas, TX, USA) at 20 ± 5°C for 8 hr or 4°C overnight in the dark.

Techniques:

Journal: Acta Histochemica et Cytochemica

Article Title: Localization of Both CD31- and Endomucin-Expressing Vessels in Mouse Dental Pulp

doi: 10.1267/ahc.24-00009

Figure Lengend Snippet: Calcein labeling and endomucin (+) vessels. ( A ) At 4 weeks after birth. Dentin with a large distance between the two calcein-lines was observed in the crown and root dentin. ( B ) Higher magnification of the boxed regions in A . Both CD31(+) and endomucin (+) vessels colored in white were thin and present directly beneath calcein-labeled dentin at all sites. ( C ) At 8 weeks after birth. Although the lines were present in root dentin, they were negligible in coronal dentin. The distance between the two lines at 8 weeks were narrower than that at 4 weeks. Both CD31 and endomucin (+) vessels were scattered in coronal dental pulp. ( D ) Higher magnification of the boxed regions in C . Both CD31 and endomucin (+) vessels were located at the odontoblast layer in root pulp. ( E ) At 12 weeks after birth. Calcein-labeled areas were only present in newly formed apical cementum, and were faint or not seen at coronal and root dentin. ( F ) Higher magnification of the boxed regions in E . CD31(+) and ndomucin (+) vessels were seen neat the newly formed cementum and faintly detected in dental pulp. Den: dentin, Cem: cementum, Ob: odontoblast layer, Pu: dental pulp. Bars = 200 μm ( A , C , E ), 30 μm ( B , D , F ). Green: calcein labeling, Cyan: CD31(+) vessel, Red: endomucin (+) vessel, White: both CD31(+) and endomucin(+) vessel, Blue: nuclear.

Article Snippet: Sections of decalcified samples were incubated with an Alexa Fluor 488-conjugated anti-CD31/PECAM-1 goat polyclonal antibody (dilution 1:100, FAB3628G-10, R&D Systems, Minneapolis, MN, USA) and a rat monoclonal antibody against mouse endomucin (dilution 1:200, sc-65495, Santa Cruz Biotechnology Inc., Dallas, TX, USA) at 20 ± 5°C for 8 hr or 4°C overnight in the dark.

Techniques: Labeling

Journal: Bioactive Materials

Article Title: 3D bioprinted multi-layered cell constructs with gradient core-shell interface for tendon-to-bone tissue regeneration

doi: 10.1016/j.bioactmat.2024.10.002

Figure Lengend Snippet: Immunohistochemical imaging of the bioprinted TBI construct in rabbit rotator cuff tear model. (A) COL1, COL3, platelet and endothelial cell adhesion molecule 1 (PECAM-1), and vascular endothelial growth factor (VEGF) immunohistochemical staining images at 8 weeks post-implantation. Semi-quantitatively estimated score of (B) COL1, (C) COL3, (D) PECAM-1, and (E) VEGF (n = 4). (F) Immunohistochemical staining images and (G) semi-quantitatively analyzed score of HLA Class 1 ABC (HLA-ABC) (n = 4). Red and blue arrows indicate the HLA-ABC-positive and -negative cells, respectively. The most efficient regenerative capacities, including tendon-to-bone tissue integration and vascular formation, were observed in the rabbit RCT model with the transplantation of the Exp bioconstructs, validated by immunohistochemical imaging analyses. All values are presented as the mean ± SD (∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.001).

Article Snippet: The sections were washed three times with PBS and incubated with primary antibodies (1:100–1:200) at room temperature for 2 h. The following primary antibodies were used: mouse anti-collagen 1 (COL1) monoclonal antibody (Abcam, Waltham, MA, USA), mouse anti-collagen 3 (COL3) monoclonal antibody (FH-7A; Abcam), goat anti-platelet endothelial cell adhesion molecule 1 (PECAM-1) polyclonal antibody (M-20; Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit anti-VEGF polyclonal antibody (A-20; Santa Cruz Biotechnology), and mouse anti-HLA Class 1 ABC (HLA-ABC; reactivity: human) monoclonal antibody (EMR8-5; Abcam).

Techniques: Immunohistochemical staining, Imaging, Construct, Staining, Transplantation Assay